PPIA antibody (Internal Region)

Catalog No. ABIN374639

Product Details anti-PPIA Antibody

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-ERFGSRNGKT SKK

Specificity: This antibody is expected to recognise Cyclophilin A. This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_066953.1. Genename: PPIA

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. Approx 18 kDa band observed in Human Lymph Node lysates(calculated MW 18.8 kDa according to NP_066953.1).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: Cyclophilin A (PPIA Products)

Background: Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.Synonyms: CYPA, Cyclophilin-A, Cyclosporin A-binding protein, EC=5.2.1.8, PPIA, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Rotamase A

Gene ID: 5478

NCBI Accession: NP_066953

UniProt: P62937