WNT3 antibody (Internal Region)

Catalog No. ABIN374685

Product Details anti-WNT3 Antibody

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes WNT3.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1. Genename: WNT3

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for WNT3

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Alternative Name: WNT3 (WNT3 Products)

Synonyms: INT4 antibody, Ci-Wnt3 antibody, WNT3 antibody, int4 antibody, Xwnt3 antibody, wnt-3 antibody, wnt3l antibody, Xwnt-3 antibody, wnt3 antibody, Int-4 antibody, Wnt-3 antibody, wnt3-A antibody, Wnt family member 3 antibody, wingless-type MMTV integration site family, member 3 antibody, proto-oncogene Wnt-3 antibody, wingless-type MMTV integration site family member 3 antibody, Wnt family member 3A antibody, wingless-type MMTV integration site family member 3 L homeolog antibody, WNT3 antibody, Wnt3 antibody, wnt3 antibody, LOC468297 antibody, WNT3A antibody, wnt3.L antibody

Background: Wnts have diverse roles in governing cell fate, proliferation, migration, and polarity during development, particularly in embryogenesis and carcinogenesis. WNTs are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity pathways. Distinct sets of Wnt and Frizzled ligand-receptor pairs activate each of these pathways and lead to unique cellular responses. Wnt3 is expressed in embryonic mouse brain (with peak expression at around day 12 of gestation) and at low levels in the adult. It is present in the cerebellum during mouse development, and is restricted to the Purkinje cell layer in the adult mouse. Wnt3 is required for normal limb development and plays an important role in the establishment of the apical ectodermal ridge. In motorneurons, Wnt3 is a retrograde signal that controls terminal branching of muscle afferents. Wnt3 is highly expressed in human cancer cell lines including A549 (lung cancer), MKN45 (gastric cancer) and MCF7 (breast cancer). Wnt3 and Wnt3a are nearly 90 % identical at the amino acid level.Synonyms: INT-4, INT4, Proto-oncogene protein Wnt-3

Gene ID: 7473, 9606

UniProt: P56703

Pathways: WNT Signaling, Regulation of Cell Size