s100a4 antibody (C-Term)

Catalog No. ABIN375119

Product Details anti-s100a4 Antibody

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: CNEFFEGFPD KQP

Specificity: This antibody recognizes S100A4 / CAPL.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Synthetic Peptide from the C-Terminus of Human S100A4 (NP_002952.1 and NP_062427.1) Genename: S100A4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/128000. Western blot: 0.03-0.1 μg/mL. Approx 13 kDa band observed in Human Placenta lysates. Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows strong nuclear staining in some cellsand weaker cytoplasm staining in some other cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 (S100A4 Products)

Synonyms: 18A2 antibody, 42A antibody, CAPL antibody, FSP1 antibody, MTS1 antibody, P9KA antibody, PEL98 antibody, 42a antibody, Capl antibody, FSp1 antibody, Mts1 antibody, PeL98 antibody, metastasin antibody, pk9a antibody, S100A4 antibody, P9ka antibody, RNP9KA antibody, S100 calcium binding protein A4 antibody, S100 calcium-binding protein A4 antibody, S100A4 antibody, S100a4 antibody

Background: S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100A4 has been implicated in motility, invasion and tubulin polymerization. It is also involved in tumor metastasis.Synonyms: CAPL, Calvasculin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1, MTS1, Metastasin, Placental Calcium-Binding Protein, S100 Calcium-Binding Protein A4, S100-A4

Molecular Weight: 11.7 kDa (NP_002952.1 and NP_062427.1).

Gene ID: 6275, 9606

UniProt: P26447

Pathways: S100 Proteins