FHL3 antibody (C-Term)

Catalog No. ABIN375133

Product Details anti-FHL3 Antibody

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FHL3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DQVLCQGCSQ AGP

Specificity: This antibody reacts to FHL3 / SLIM2.

Cross-Reactivity (Details): Species reactivity (expected):Porcine, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_004459.2.

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000Western Blot: 1-3 μg/mL. Approx 30 kDa band observed in Human, Mouse and Rat SkeletalMuscle lysates (calculated MW of 31.0 kDa according to NP_004459.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FHL3

Target: FHL3 (Four and A Half LIM Domains 3 (FHL3))

Alternative Name: FHL3 (FHL3 Products)

Synonyms: slim2 antibody, MGC79794 antibody, fb09g09 antibody, wu:fb09g09 antibody, zgc:63514 antibody, fd57e01 antibody, fhl3 antibody, wu:fd57e01 antibody, SLIM2 antibody, four and a half LIM domains 3 antibody, four and a half LIM domains 3 S homeolog antibody, four and a half LIM domains 3b antibody, four and a half LIM domains 3a antibody, FHL3 antibody, fhl3.S antibody, fhl3 antibody, fhl3b antibody, fhl3a antibody, Fhl3 antibody

Background: FHL3 interacts with human phosphatase CDC25B in the cell nucleus. LIM domain proteins such as FHL3 are found to be important regulators in cell growth, cell fate determination, cell differentiation and remodeling of the cell cytoskeleton. Human FHL3 is a type of LIM only protein that contains four tandemly repeated LIM motifs with an N terminal single zinc finger (half LIM motif).Synonyms: FHL-3, Four and a half LIM domains protein 3, SLIM2, Skeletal muscle LIM-protein 2

Gene ID: 2275, 9606

UniProt: Q13643