NPC2 antibody (Internal Region)

Catalog No. ABIN498021

Product Details anti-NPC2 Antibody

Target: NPC2 (Niemann-Pick Disease, Type C2 (NPC2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NPC2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: QAEPVQFKDC GSVD

Specificity: Recognizes Niemann-Pick disease type C2 NPC2.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006423.1. Genename: NPC2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/2000. Western blot: 0.3-1 μg/mL. Experiments gave bands at approx 20 kDa and 20 kDa in HumanTestis lysates. These bands correspond to earlier findings in literature with differentantibodies (Ref.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NPC2

Target: NPC2 (Niemann-Pick Disease, Type C2 (NPC2))

Alternative Name: NPC2 (NPC2 Products)

Synonyms: 2700012J19Rik antibody, AA408070 antibody, AU045843 antibody, HE1 antibody, EDDM1 antibody, re1 antibody, CE1 antibody, EPI-1 antibody, cb292 antibody, sb:cb292 antibody, NPC intracellular cholesterol transporter 2 antibody, Niemann-Pick disease, type C2 antibody, Npc2 antibody, NPC2 antibody, npc2 antibody

Background: Niemann-Pick C2 (NPC2) is a 22 kDa soluble lysosomal glycoprotein important for cholesterol and sphingolipid transport in the lysosome. This protein was initially identified as the second Niemann-Pick type C (NPC) gene based on its ability to bind the mannose-6 phosphate receptor. NPC disease is an autosomal recessively inherited neurodegenerative lysosomal storage disease and its associated with abnormal cholesterol accumulation in cells. Mutations in the NPC2 gene cause 5 % of the cases, the rest and majority being caused by NPC1 mutations.Synonyms: Epididymal secretory protein E1

Gene ID: 10577

NCBI Accession: NP_006423

UniProt: P61916

Pathways: SARS-CoV-2 Protein Interactome