POU3F2 antibody (Internal Region)

Catalog No. ABIN498033

Product Details anti-POU3F2 Antibody

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-AQSLVQGDYG ALQSN

Specificity: Recognizes POU3F2/BRN2/OCT7.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine and Porcine.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_005595.2 Genename: POU3F2

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-3 μg/mL. Approx 45 kDa band observed in Human Brain (Cerebellum) andin Mouse and Rat Brain lysates (calculated MW of 46.5 kDa according to HumanNP_005595.2 and 47.5 kDa according to Mouse NP_032925.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2 (POU3F2 Products)

Synonyms: BRN2 antibody, N-Oct3 antibody, OCT7 antibody, OTF-7 antibody, OTF7 antibody, POUF3 antibody, brn-2 antibody, oct-7 antibody, XlPOU3 antibody, pou3f2 antibody, pou3f2-A antibody, pou3f4 antibody, 9430075J19Rik antibody, A230098E07Rik antibody, Brn-2 antibody, Brn2 antibody, Otf7 antibody, XLPOU3b antibody, pou47 antibody, zp47 antibody, zp47pou antibody, brn-1.2 antibody, brn1.2 antibody, id:ibd5088 antibody, POU class 3 homeobox 2 antibody, POU class 3 homeobox 2 L homeolog antibody, POU domain, class 3, transcription factor 2 antibody, POU class 3 homeobox 2 S homeolog antibody, POU class 3 homeobox 2b antibody, POU class 3 homeobox 2a antibody, POU3F2 antibody, pou3f2.L antibody, Pou3f2 antibody, pou3f2.S antibody, pou3f2b antibody, pou3f2a antibody

Background: POU3F2 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.Synonyms: BRN2, Brain-2, Brain-specific homeobox/POU domain protein 2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, OCT7, OTF-7, OTF7, Oct-7, Octamer-binding protein 7, Octamer-binding transcription factor 7, POU domain class 3 transcription factor 2

Gene ID: 5454

NCBI Accession: NP_005595

UniProt: P20265