SOX3 antibody (C-Term)

Catalog No. ABIN498075

Product Details anti-SOX3 Antibody

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This SOX3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-DAASPLPGGR LHGVH

Specificity: This antibody recognizes SOX3 (C-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide corresponding to the C-Terminus of Human SOX3 (NP_005625.2). Genename: SOX3

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 42 kDa in Human Brain (Cerebellum, Cerebral Cortex and FrontalCortex) lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog). Add. Information: Calculated Molecular Weight: 45.2 kDa according to NP_005625.2

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SOX3

Target: SOX3 (SRY (Sex Determining Region Y)-Box 3 (SOX3))

Alternative Name: SOX3 (SOX3 Products)

Synonyms: GHDX antibody, MRGH antibody, PHP antibody, PHPX antibody, SOXB antibody, CH3 antibody, Sry antibody, Xlsox3 antibody, Xtsox3 antibody, mrgh antibody, soxb antibody, xSox3 antibody, Sox-3 antibody, fc93f07 antibody, id:ibd2036 antibody, sb:cb493 antibody, wu:fc93f07 antibody, wu:fd02a08 antibody, zgc:110279 antibody, sox3-a antibody, xSox-B1 antibody, SRY-box 3 antibody, transcription factor SOX-3 antibody, SRY (sex determining region Y)-box 3 antibody, SRY-box 3 S homeolog antibody, SOX3 antibody, Tsp_06143 antibody, sox3 antibody, Sox3 antibody, sox3.S antibody

Background: SOX3 is also known as SRY related HMG BOX gene 3. All SOX proteins have a single HMG box. Humans with mutations in SOX3 have panhypopituitarism. Conditional disruption of SOX3 in mice demonstrates that anterior pituitary development depends on SOX3 expression in the overlying neural ectoderm.Synonyms: GHDX, MRGH, SOXB, SRY (sex determining region Y)-box 3, SRY-box 3, Transcription factor SOX-32

Molecular Weight: 45.2kDa according to NP_005625.2

Gene ID: 6658

NCBI Accession: NP_005625

UniProt: P41225