NDUFS8 antibody (Internal Region)

Catalog No. ABIN499135

Product Details anti-NDUFS8 Antibody

Target: NDUFS8 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8, 23kDa (NADH-Coenzyme Q Reductase) (NDUFS8))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-EAEIAANIQA D

Specificity: Recognizes NDUFS8.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002487.1. Genename: NDUFS8

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 25 kDa in Mouse and RatHeart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS8

Target: NDUFS8 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8, 23kDa (NADH-Coenzyme Q Reductase) (NDUFS8))

Alternative Name: NDUFS8 (NDUFS8 Products)

Synonyms: CI-23k antibody, CI23KD antibody, TYKY antibody, BC021616 antibody, CI-23kD antibody, ndufs8 antibody, wu:fb30b07 antibody, zgc:65835 antibody, zgc:77840 antibody, MGC85457 antibody, ND23 antibody, GB17476 antibody, DDBDRAFT_0169117 antibody, DDBDRAFT_0233205 antibody, DDB_0169117 antibody, DDB_0233205 antibody, zgc:109991 antibody, NADH:ubiquinone oxidoreductase core subunit S8 antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8 antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8a antibody, NADH:ubiquinone oxidoreductase core subunit S8 L homeolog antibody, NADH-quinone oxidoreductase subunit I antibody, NADH dehydrogenase Fe-S protein 8 antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase) antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 8b antibody, NDUFS8 antibody, Ndufs8 antibody, ndufs8a antibody, ndufs8.L antibody, ndufs8 antibody, LOC551078 antibody, ndufs8b antibody

Background: NDUFS8 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (may donate electrons to ubiquinone). Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.Synonyms: CI-23kD, Complex I-23kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, mitochondrial

Molecular Weight: 24.0kDa (Mouse NP_659119.2 and Rat NP_001099792.1).

Gene ID: 4728

NCBI Accession: NP_002487

UniProt: O00217