PHLDA3 antibody (C-Term)

Catalog No. ABIN615985

Product Details anti-PHLDA3 Antibody

Target: PHLDA3 (Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PHLDA3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-RARQSLGTGT LVS

Specificity: Recognizes PHLDA3 / TIH1

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: synthetic peptide corresponding to the C Terminus of the protein sequence of Huuman PHLDA3 (NP_036528.1). Genename: PHLDA3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PHLDA3

Target: PHLDA3 (Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3))

Alternative Name: PHLDA3 (PHLDA3 Products)

Synonyms: TIH1 antibody, Tih1 antibody, si:dz182n13.5 antibody, zgc:92333 antibody, pleckstrin homology like domain family A member 3 antibody, pleckstrin homology like domain, family A, member 3 antibody, pleckstrin homology-like domain, family A, member 3 antibody, PHLDA3 antibody, Phlda3 antibody, phlda3 antibody

Background: P53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling.Synonyms: Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homologue 1, TIH1

Gene ID: 23612

NCBI Accession: NP_036528

UniProt: Q9Y5J5