Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RFX5 antibody (AA 320-494)

RFX5 Reactivity: Human, Mouse WB, IP, EIA, EMSA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN116532
  • Target See all RFX5 Antibodies
    RFX5 (Regulatory Factor X 5 (RFX5))
    Binding Specificity
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 320-494
    Reactivity
    • 35
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    Human, Mouse
    Host
    • 32
    • 3
    • 1
    Rabbit
    Clonality
    • 34
    • 2
    Polyclonal
    Conjugate
    • 27
    • 2
    • 2
    • 2
    • 2
    • 2
    This RFX5 antibody is un-conjugated
    Application
    • 34
    • 20
    • 6
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)
    Specificity
    Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa. Human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is of the region between amino acids 320 to 494. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.
    Purification
    Prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
    Immunogen
    RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
    Top Product
    Discover our top product RFX5 Primary Antibody
  • Application Notes
    Suitable for for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed byimmunoblot and found to be reactive against RFX5 (aa 320 to494) from a variety of fibroblast and B-cell lysates at a dilution of 1: 1,000 followed byreaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot for human RFX5. This product was also tested in a gel supershift assay and found to be reactive againstRFX5 complexes using 0.5 to 1.0 l per assay.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.68 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at -20 °C.
  • Target
    RFX5 (Regulatory Factor X 5 (RFX5))
    Alternative Name
    RFX5 (RFX5 Products)
    Synonyms
    regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody
    Background
    RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5
    Gene ID
    5993
    NCBI Accession
    NP_000440
    UniProt
    P48382
You are here:
Support