Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN116600

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Alkaline Phosphatase [Human Intestine].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: Alkaline Phosphatase / ALPI / IAP (ALPI Products)

Synonyms: IAP antibody, Alp1 antibody, S51097 antibody, 2010001C14Rik antibody, Akp6 antibody, gIAP antibody, alpi antibody, im:7149588 antibody, si:ch211-262h13.1 antibody, wu:fa95f04 antibody, zgc:110794 antibody, ALPI antibody, Alpi antibody, alkaline phosphatase, intestinal antibody, alkaline phosphatase, intestinal, tandem duplicate 1 antibody, intestinal-type alkaline phosphatase antibody, ALPI antibody, Alpi antibody, alpi.1 antibody, LOC100724954 antibody

Background: Synonyms: Intestinal alkaline phosphatase

Gene ID: 248

NCBI Accession: NP_001622

UniProt: P09923