Recombinant Rab6-GTP (Gln72Leu-Mutant) antibody (Atto 488)

Catalog No. ABIN5564232

Product Details

Target: Rab6-GTP

Antibody Type: Recombinant Antibody

Binding Specificity: Gln72Leu-Mutant

Reactivity: Drosophila melanogaster, Human, Mouse

Host: Human

Clonality: Monoclonal

Conjugate: Atto 488

Application: Immunocytochemistry (ICC)

Specificity: Recognizes human, mouse and Drosophila GTP-bound Rab6a and Rab6b and mutant Rab6Q72L.

Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine)

Cross-Reactivity (Details): Does not detect Rab6•GDP.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant Rab6AQ72L, a GTP-locked mutant of Rab6A in which Gln72 is replaced by Leu.

Clone: AA2

Isotype: IgG2

Application Details

Comment:

anti-Rab6-GTP, monoclonal antibody (recombinant) (AA2) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Rab6-GTP, monoclonal antibody (recombinant) (AA2) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: +4°C
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C.

Expiry Date: 12 months

Target Details

Target: Rab6-GTP

Background: Rab6 is involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).

UniProt: P20340