BRAF antibody

Catalog No. ABIN5573331

Product Details anti-BRAF Antibody

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit polyclonal antibody raised against synthetic peptide of BRAF.

Specificity: This antibody is specific to BRAF.

Cross-Reactivity: Human, Mouse

Immunogen: A synthetic peptide corresponding to residues surrounding S446 of human BRAF.

Application Details

Application Notes: Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS, 150 mM NaCl, pH 7.4 (50 % glycerol, 0.02 % sodium azide)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.

References for anti-BRAF antibody (ABIN5573331)

Rushton, Korb, Kummer, Reichart, Fuchs-Baumgartinger, Tichy, Nell: "Protein expression of KIT, BRAF, GNA11, GNAQ and RASSF1 in feline diffuse iris melanomas." in: Veterinary journal (London, England : 1997), Vol. 249, pp. 33-40, (2019) (PubMed).

Wiggans, Reilly, Kass, Maggs: "Histologic and immunohistochemical predictors of clinical behavior for feline diffuse iris melanoma." in: Veterinary ophthalmology, Vol. 19 Suppl 1, pp. 44-55, (2016) (PubMed).

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF (BRAF Products)

Synonyms: B-RAF1 antibody, BRAF1 antibody, NS7 antibody, RAFB1 antibody, C-RMIL antibody, RMIL antibody, B-Raf antibody, braf1 antibody, rafb1 antibody, b-raf1 antibody, sierrabraf antibody, BRAF antibody, 9930012E13Rik antibody, AA120551 antibody, AA387315 antibody, AA473386 antibody, B-raf antibody, Braf-2 antibody, Braf2 antibody, C230098H17 antibody, C87398 antibody, D6Ertd631e antibody, braf antibody, fc48g01 antibody, wu:fc48g01 antibody, B-Raf proto-oncogene, serine/threonine kinase antibody, B-Raf proto-oncogene, serine/threonine kinase S homeolog antibody, Braf transforming gene antibody, si:dkey-222f8.3 antibody, BRAF antibody, Braf antibody, braf.S antibody, braf antibody, si:dkey-222f8.3 antibody

Background: Full Gene Name: v-raf murine sarcoma viral oncogene homolog B1
Synonyms: B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1

Gene ID: 673

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy