CAS9 (C-Term) antibody

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Antigen
Epitope
C-Term
2
1
1
Reactivity
Streptococcus pyogenes (S. pyogenes)
8
1
Host
Rabbit
6
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
Application
ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
9
4
4
3
2
2
1
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Immunogen

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Peptide

Isotype IgG
Purification Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Alternative Name Cas 9
Background

Synonyms: CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, csn1, Cas-9 Antibody

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gene Name: cas9

Gene ID 901176
UniProt Q99ZW2
Application Notes

Application Note: This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:25,000-1:30,000

IF Microscopy Dilution: 1 μg/mL

Comment

User Optimized

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months
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