CAS9 (C-Term) antibody (Biotin)

Details for Product No. ABIN5596702, Supplier: Log in to see
Antigen
Epitope
C-Term
2
1
1
Reactivity
Streptococcus pyogenes (S. pyogenes)
8
1
Host
Rabbit
6
3
Clonality
Polyclonal
Conjugate
Biotin
1
Application
ELISA, Fluorescence Microscopy (FM), Western Blotting (WB)
9
4
4
3
2
2
1
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Immunogen

Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

Immunogen Type: Peptide

Isotype IgG
Purification Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Alternative Name Cas 9
Background

Synonyms: CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, csn1, Biotin conjugation, Cas-9 Antibody

Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

Gene Name: cas9

Gene ID 901176
NCBI Accession NP_269215
UniProt Q99ZW2
Application Notes

Application Note: Cas9 Biotin Conjugated Antibody has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:25,000-1:30,000

IF Microscopy Dilution: 1 μg/mL

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months