V5 Epitope Tag antibody

Details for Product No. ABIN559686, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Mouse
220
135
37
9
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
29
27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunostaining (ISt), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
342
265
123
91
71
65
53
36
28
13
9
8
4
4
4
2
2
1
1
1
1
1
Options
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Immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Sequence GKPIPNPLLG LDST
Clone E10
Isotype IgG1
Specificity Recognizes V5-tagged fusion proteins
Sensitivity WB (with ECL): 1-2ng of V5-tagged fusion proteins
Purification Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Alternative Name V5-Tag
Target Type Tag
Research Area Tags/Labels
Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Restrictions For Research Use only
Concentration 1mg/mL
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-V5 Epitope Tag antibody (ABIN559686) 1:000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged pro...