Flavivirus Group Antigen antibody

Details for Product No. ABIN5608868, Supplier: Log in to see
Antigen
Reactivity
Flavivirus
15
1
1
Host
Mammalian Cells
15
2
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunofluorescence (IF), Neutralization (Neut), Western Blotting (WB)
16
15
14
2
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose Monoclonal antibody to detect Flavivirus group antigen.
Immunogen Dengue Virus type 2 antigens
Clone D1-4G2-4-15 (4G2)
Isotype IgG, kappa
Purification Affinity purified using protein A
Background This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation. The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004. Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008).
Synonyms: N/A
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Suggested positive control:
IF: Vero cells infected with ZIKV

Restrictions For Research Use only
Format Liquid
Buffer 200 μg affinity purified antibody in phosphate-buffered saline (PBS) containing 0.02 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use
Storage -20 °C
Expiry Date 12 months
Did you look for something else?