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Separase antibody (AA 586-701)

ESPL1 Reactivity: Human ELISA, WB, IHC (p) Host: Mouse Monoclonal 6H6 unconjugated
Catalog No. ABIN564183
  • Target See all Separase (ESPL1) Antibodies
    Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))
    Binding Specificity
    • 18
    • 15
    • 15
    • 9
    • 6
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 586-701
    Reactivity
    • 94
    • 11
    • 1
    Human
    Host
    • 89
    • 5
    • 2
    Mouse
    Clonality
    • 91
    • 5
    Monoclonal
    Conjugate
    • 32
    • 9
    • 9
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This Separase antibody is un-conjugated
    Application
    • 46
    • 38
    • 26
    • 26
    • 21
    • 14
    • 11
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Mouse monoclonal antibody raised against a partial recombinant ESPL1.
    Sequence
    REELQAYKAV RADTGQERFN IICDLLELSP EETPAGAWAR ATHLVELAQV LCYHDFTQQT NCSALDAIRE ALQLLDSVRP EAQARDQLLD DKAQALLWLY ICTLEAKIQE GIERDR
    Cross-Reactivity
    Human
    Characteristics
    Antibody Reactive Against Recombinant Protein.
    Immunogen
    ESPL1 (NP_036423, 586 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
    Clone
    6H6
    Isotype
    IgG2a
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    In 1x PBS, pH 7.4
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Mukherjee, Ge, Zhang, Edwards, Sumazin, Sharan, Rao, Medina, Pati: "MMTV-Espl1 transgenic mice develop aneuploid, estrogen receptor alpha (ER?)-positive mammary adenocarcinomas." in: Oncogene, Vol. 33, Issue 48, pp. 5511-22, (2014) (PubMed).

    Mukherjee, Byrd, Brawley, Bielamowicz, Li, Merchant, Maitra, Sumazin, Fuller, Kew, Sun, Powell, Ahmed, Zhang, Pati: "Overexpression and constitutive nuclear localization of cohesin protease Separase protein correlates with high incidence of relapse and reduced overall survival in glioblastoma multiforme." in: Journal of neuro-oncology, Vol. 119, Issue 1, pp. 27-35, (2014) (PubMed).

    Zhang, Scorsone, Ge, Kaffes, Dobrolecki, Mukherjee, Lewis, Berg, Stephan, Pati: "Identification and Characterization of Separase Inhibitors (Sepins) for Cancer Therapy." in: Journal of biomolecular screening, Vol. 19, Issue 6, pp. 878-889, (2014) (PubMed).

    Mukherjee, Ge, Zhang, Huang, Nakamura, Minor, Fofanov, Rao, Herron, Pati: "Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice." in: PLoS ONE, Vol. 6, Issue 7, pp. e22167, (2011) (PubMed).

    Panigrahi, Zhang, Mao, Pati: "Calpain-1 cleaves Rad21 to promote sister chromatid separation." in: Molecular and cellular biology, Vol. 31, Issue 21, pp. 4335-47, (2011) (PubMed).

    Meyer, Fofanov, Panigrahi, Merchant, Zhang, Pati: "Overexpression and mislocalization of the chromosomal segregation protein separase in multiple human cancers." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2703-10, (2009) (PubMed).

    Zhang, Ge, Meyer, Sethi, Basu, Pradhan, Zhao, Li, Cai, El-Naggar, Baladandayuthapani, Kittrell, Rao, Medina, Pati: "Overexpression of Separase induces aneuploidy and mammary tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 35, pp. 13033-8, (2008) (PubMed).

  • Target
    Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))
    Alternative Name
    ESPL1 (ESPL1 Products)
    Synonyms
    ESP1 antibody, SEPA antibody, mp:zf637-3-001691 antibody, AL024103 antibody, AU045071 antibody, Cerp antibody, PRCE antibody, SSE antibody, extra spindle pole bodies like 1, separase antibody, extra spindle poles like 1 antibody, extra spindle pole bodies 1, separase antibody, ESPL1 antibody, espl1 antibody, Espl1 antibody
    Background
    Full Gene Name: extra spindle pole bodies homolog 1 (S. cerevisiae)
    Synonyms: ESP1,FLJ46492,KIAA0165,SEPARASE,SEPARIN
    Gene ID
    9700
    NCBI Accession
    NM_012291
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