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Collagen, Type I (COL1) antibody

Details for Product No. ABIN567631, Supplier: Login to see
Antigen
Reactivity
Cow (Bovine), Deer, Human, Pig (Porcine), Rabbit, Rat (Rattus)
188
62
54
25
18
10
9
8
7
6
4
3
2
2
2
2
2
1
1
Host
Mouse
194
55
41
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
32
7
5
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
192
104
100
86
83
71
69
42
29
28
25
15
14
8
5
4
4
2
1
1
1
1
Supplier
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Clone COL-1
Isotype IgG1
Specificity Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The antibody shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.
Purification Supernatant
Alternative Name Collagen Type I
Background Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Gene ID 1277
Research Area Extracellular Matrix
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered saline, pH 7.2, no preservative added.
Preservative Without preservative
Supplier Images
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN567631) Staining of Acetone-Fixed, Frozen Sections of Human tongue with 1/2,000 Monoclonal An...
Immunofluorescence (IF) image for anti-Collagen, Type I (COL1) antibody (ABIN567631) Staining of Acetone-Fixed, Frozen Sections of Pig tongue with 1/4,000 Monoclonal Anti...
Background publications Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

Mayne: "Preparation and applications of monoclonal antibodies to different collagen types." in: Clinical biochemistry, Vol. 21, Issue 2, pp. 111-5, 1988 (PubMed).

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).