Collagen Type I antibody

Details for Product No. ABIN567631
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Antigen
Reactivity
Cow (Bovine), Deer, Human, Pig (Porcine), Rabbit, Rat (Rattus)
(32), (14), (8), (8), (7), (7), (4), (4), (4), (4), (2), (2), (2), (2), (1), (1), (1)
Host
Mouse
(45), (10), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)
(42), (38), (36), (29), (28), (21), (14), (10), (6), (6), (3), (3), (1)
Pubmed 4 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN567631
390.50 $
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Clone COL-1
Isotype IgG1
Specificity Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The antibody shows no cross-reactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI. Strong staining of connective tissue fibres is seen in acetone-fixed or unfixed frozen sections.
Purification Supernatant
Background Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Gene ID 1277
Research Area Extracellular Matrix
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Phosphate-buffered saline, pH 7.2, no preservative added.
Preservative Without preservative
Background publications Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

Werkmeister, Ramshaw: "Monoclonal antibodies to collagens for immunofluorescent examination of human skin." in: Acta dermato-venereologica, Vol. 69, Issue 5, pp. 399-402, 1989 (PubMed).

Mayne: "Preparation and applications of monoclonal antibodies to different collagen types." in: Clinical biochemistry, Vol. 21, Issue 2, pp. 111-5, 1988 (PubMed).

Gay, Fine: "Characterization and isolation of poly- and monoclonal antibodies against collagen for use in immunohistochemistry." in: Methods in enzymology, Vol. 145, pp. 148-67, 1987 (PubMed).

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