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Cytochrome C antibody (Cytochrome C, Somatic)

Details for Product anti-CYCS Antibody No. ABIN567867, Supplier: Login to see New
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Human, Mouse (Murine), Rat (Rattus)
(191), (108), (98), (49), (23), (19), (18), (10), (9), (9), (8), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(147), (118), (7), (4)
Clonality (Clone)
Monoclonal ()
This Cytochrome C antibody is un-conjugated
(17), (9), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP)
(191), (74), (61), (51), (49), (49), (37), (19), (15), (11), (11), (6), (6), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mg
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Immunogen Rat Cytochrome c was used as immunogen to generate 6H2.B4 hybridoma clone (Goshorn et al, 1991).
Clone 6H2-B4
Isotype IgG1
Specificity Recognizes Cytochrome C. This antibody recognizes an epitope around residue 62 of Rat Cytochrome c, based on copetitive ELISA results (Goshorn et al. 1991). Clone 6H2.B4 (AM20329PU-N) recognizes the native form of Cytochrome c (Goshorn et al. 1991). Native Cytochrome c has an approximate molecular weight of 15 kDa (2). Clone 6H2. B4 does not appear to recognize the denatured form of Cytochrome c. Blocking: Clone 6H2.B4 has been shown to inhibit caspase 3 activation when microinjected into cells.
Purification Protein G Chromatography
Alternative Name Cytochrome c (CYCS Antibody Abstract)
Background Addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).
Alternate names: CYC, CYCS
Gene ID 54205
NCBI Accession NP_061820
UniProt P99999
Research Area Apoptosis/Necrosis, Metabolism
Pathways Apoptosis, Caspase Cascade in Apoptosis
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% Gelatin and 0.05% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Cytochrome C antibody (Cytochrome C, Somatic) (ABIN567867) anti-Cytochrome C, Somatic (CYCS) antibody
Background publications Zou, Henzel, Liu et al.: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Liu, Kim, Yang et al.: "Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c." in: Cell, Vol. 86, Issue 1, pp. 147-57, 1996 (PubMed).

Fernandes-Alnemri, Litwack, Alnemri: "CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme." in: The Journal of biological chemistry, Vol. 269, Issue 49, pp. 30761-4, 1995 (PubMed).

Catalog No. ABIN567867

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