Glutathione Reductase antibody (Biotin)

Catalog No. ABIN568226

Product Details anti-Glutathione Reductase Antibody (Biotin)

Target: Glutathione Reductase (GSR)

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Reductase antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Immunodiffusion (ID), Dot Blot (DB)

Specificity: The antibody recognizes Glutathione Reductase from Baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~6.2

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Glutathione Reductase isolated and purified from Baker's Yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: Glutathione Reductase (GSR Products)

Synonyms: GR antibody, ATGR2 antibody, EMB2360 antibody, glutathione reductase antibody, AI325518 antibody, D8Ertd238e antibody, Gr-1 antibody, Gr1 antibody, DDBDRAFT_0168952 antibody, DDBDRAFT_0231410 antibody, DDB_0168952 antibody, DDB_0231410 antibody, 2151 antibody, CG2151 antibody, DTR antibody, Dm-TrxR antibody, DmTR antibody, DmTrx antibody, DmTrxR antibody, DmTrxR-1 antibody, Dmel\\CG2151 antibody, Gr antibody, Trx antibody, TrxR antibody, TrxR-1 antibody, Trxr1 antibody, anon-WO03040301.185 antibody, anon-WO03040301.187 antibody, dTrxR antibody, dmtrxr-1 antibody, gr antibody, l(1)G0154 antibody, l(1)G0379 antibody, l(1)G0477 antibody, l(1)G0481 antibody, trxr-1 antibody, gor1 antibody, glutathione reductase, gro-2 antibody, glutathione reductase antibody, glutathione-disulfide reductase antibody, GR; GRase antibody, mitochondrial glutathione reductase Pgr1 antibody, Glutathione reductase (GR) (GRase) antibody, Thioredoxin reductase-1 antibody, glutathione-disulfide reductase family protein antibody, glutathione reductase 1 antibody, GR antibody, gor antibody, GSR antibody, GSR1 antibody, Gsr_2 antibody, Gsr antibody, GSHR1 antibody, gsr antibody, LbGR antibody, pgr1 antibody, GSHR2 antibody, GLR2 antibody, Bcen_2392 antibody, RPE_1989 antibody, HCAG_02219 antibody, SJAG_01162 antibody, Trxr-1 antibody, PF14_0192 antibody, POPTR_0001s14480g antibody, gsr1 antibody

Background: Glutathione reductase (GR) is a member of pyridine nucleotide-disulfideoxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione(GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG): GSSG + NADPH + H+ ?2GSH + NADP+. In mosteukaryotic cells, GR maintains the ratio of [GSH]/[GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.Synonyms: GLUR, GR, GRD1, GRase, GSR

Gene ID: 856014

NCBI Accession: NP_015234

UniProt: P41921

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis