Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
Specificity
Affinity purified antibodies of polyclonal rabbit antiserum to 3-phosphoglyceric phosphokinase from baker's yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Purification
Solid Phase Affinity Chromatography
Immunogen
3-Phosphoglyceric phosphokinase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.