Western Blotting (WB), Immunofluorescence (IF), Immunodiffusion (ID), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB)
Specificity
Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase A2 from porcine pancreas. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Characteristics
Molar Ratio: Biotin/IgG ~6.1
Purification
Ammonium Sulphate Precipitation and Ion Exchange Chromatography
Immunogen
Phospholipase A2 isolated and purified from porcine pancreas. Freund’s complete adjuvant is used in the first step of the immunization procedure.