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C3 antibody (FITC)

C3 Reactivity: Guinea Pig WB, IP, EIA, IF, ID, DB Host: Goat Polyclonal FITC
Catalog No. ABIN568553
  • Target See all C3 Antibodies
    C3 (Complement Component 3 (C3))
    Reactivity
    • 94
    • 57
    • 35
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    Guinea Pig
    Host
    • 72
    • 46
    • 28
    • 8
    • 7
    • 6
    Goat
    Clonality
    • 128
    • 38
    Polyclonal
    Conjugate
    • 75
    • 24
    • 23
    • 15
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This C3 antibody is conjugated to FITC
    Application
    • 109
    • 54
    • 39
    • 35
    • 33
    • 30
    • 29
    • 29
    • 22
    • 18
    • 14
    • 13
    • 13
    • 9
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunodiffusion (ID), Dot Blot (DB)
    Specificity
    In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of guinea pig serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of guinea pig plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
    Characteristics
    Absorption / Emission: 492 nm / 515 nm.
    Molar Ratio: Fluorochrome/IgG ~1.6
    Purification
    Hyperimmune antisera with strong precipitating activity are selected for Fractionation and purification of the IgG fraction containing the bulk of the defined antibody specificity.
    Immunogen
    C3c is isolated and purified from pooled normal guinea pig serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1 mL of sterile distilled water
    Concentration
    10,0 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
  • Target
    C3 (Complement Component 3 (C3))
    Alternative Name
    Complement C3 (C3 Products)
    Synonyms
    AHUS5 antibody, ARMD9 antibody, ASP antibody, C3a antibody, C3b antibody, CPAMD1 antibody, AI255234 antibody, HSE-MSF antibody, Plp antibody, C3 antibody, C3-1 antibody, sb:cb26 antibody, si:dkey-76b14.4 antibody, wu:fi34b03 antibody, wu:fj86e11 antibody, C3d antibody, asp antibody, cpamd1 antibody, c3 antibody, complement C3 antibody, complement component 3 antibody, complement component c3a, duplicate 1 antibody, complement component 3 L homeolog antibody, complement component C3 antibody, complement C3-like antibody, C3 antibody, c3a.1 antibody, c3.L antibody, LOC443475 antibody, c3 antibody, c3-1 antibody, LOC100060539 antibody
    Background
    C3 is the most abundant complement protein in guinea pig serum. Its biological function strongly resembles that of C3 in man an other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response. The antiserum is raised against C3c, which is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. Consequently the antiserum reacts with both native and activated C3. It may also react with the fragments C3b, C3bi and C3dg, since they all carry antigenic epitopes of the C3c domain.Synonyms: CPAMD1, Complement component 3
    Gene ID
    24232
    NCBI Accession
    NP_058690
    UniProt
    P01026
    Pathways
    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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