Zinc Finger Protein GLI1 (GLI1) (AA 940-990) antibody

Details for Product No. ABIN569040
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Antigen
Epitope
AA 940-990
(3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Monkey
(45), (19), (15), (15), (13), (6), (1), (1)
Host
Rabbit
(44), (7), (4), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(40), (19), (10), (7), (6), (6), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN569040
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Immunogen A portion of amino acids 940-990 of Human GLI1 .Remarks: 100% homologous in Human and Rhesus Monkey 94% homologous in Horse 88% homologous in Dog 76% homologous in Cow, Mouse and Rat
Specificity This antibody recognizes GLI1. Species Reactivity: Tested: Human, Rhesus Monkey. Expected from sequence similarity: Mouse, Rat, Horse, Dog, Cow.
Purification Protein A Chromatography
Alternative Name GLI1
Background GLI1 is a protein originally isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was originally isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing GLI1 may arise from hair follicles. The level of GLI1 expression correlates with the tumor grade in bone and soft tissue sarcomas. GLI1 expression can be cytoplasm and or nucleus. The GLI1 protein is tethered in the cytoplasm by binding to SUFU. Activation and translocation of the GLI1 protein to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is also promoted by interaction with ZIC1.
Alternate names: GLI, GLI-1, Glioma-associated oncogene, Oncogene GLI, Zinc finger protein GLI1
Gene ID 2735
NCBI Accession NP_001153517
UniProt P08151
Research Area Growth Factors
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS, 0.05% Sodium Azide 0.2% Gelatin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Dahmane, Lee, Robins et al.: "Activation of the transcription factor Gli1 and the Sonic hedgehog signalling pathway in skin tumours." in: Nature, Vol. 389, Issue 6653, pp. 876-81, 1997 (PubMed).

Dunaeva, Michelson, Kogerman et al.: "Characterization of the physical interaction of Gli proteins with SUFU proteins." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 5116-22, 2003 (PubMed).

Callahan, Ofstad, Horng et al.: "MIM/BEG4, a Sonic hedgehog-responsive gene that potentiates Gli-dependent transcription." in: Genes & development, Vol. 18, Issue 22, pp. 2724-9, 2004 (PubMed).

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