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Histone H3.3 (AA 100-150) antibody

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AA 100-150
(8), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Chicken, Fruit Fly (Drosophila melanogaster), Horse (Equine), Human, Mouse (Murine), Opossum (Didelphimorphia)
(58), (12), (11), (2), (2), (1)
(57), (4)
(8), (8), (8)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(41), (34), (13), (11), (6), (6), (3), (2), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mg
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Immunogen A portion of amino acids 100-150 of human Histone H3.3 .Remarks: 100% homologous in human (Histone H3.3A, B, C, D and H3.3E), Drosophila, boar, orangutan, horse, platypus, mouse, rat, cow, chicken, Xenopus, salmon and zebrafish
Specificity This antibody reacts to Histone H3.3.
Purification Protein A chromatography
Background Histone H3.3 is a nuclear protein and a replication-independent member of the histone H3 family that has a role in nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H3.3 constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. It has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Expression of H3F3A is common throughout the cell cycle independent of DNA synthesis.
Alternate names: H3.3A, H3F3, H3F3A, PP781
Gene ID 3020, 9606
UniProt P84243
Restrictions For Research Use only
Format Liquid
Concentration 0,5 mg/mL
Buffer PBS containing 0.05 % BSA and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Expiry Date 12 months
Background publications Guccione, Bassi, Casadio et al.: "Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive." in: Nature, Vol. 449, Issue 7164, pp. 933-7, 2007 (PubMed).

Cha, Zhou, Xia et al.: "Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3." in: Science (New York, N.Y.), Vol. 310, Issue 5746, pp. 306-10, 2005 (PubMed).

Anest, Hanson, Cogswell et al.: "A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression." in: Nature, Vol. 423, Issue 6940, pp. 659-63, 2003 (PubMed).

Hall, Shankaranarayana, Noma et al.: "Establishment and maintenance of a heterochromatin domain." in: Science (New York, N.Y.), Vol. 297, Issue 5590, pp. 2232-7, 2002 (PubMed).

Catalog No. ABIN569076

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