Methyl-CpG Binding Domain Protein 4 (MBD4) (AA 268-282), (AA 337-352) antibody

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Antigen
Synonyms MED1, Med1
Epitope
AA 268-282, AA 337-352
(1), (1), (1), (1)
Reactivity
Human
(15), (5), (2)
Host
Rabbit
(12), (3)
Clonality
Polyclonal
Application
Western Blotting (WB)
(15), (6), (1), (1)
Pubmed 4 references available
Catalog no. ABIN569178
Quantity 0.1 mg
Price
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Immunogen Mixture of synthetic peptides corresponding to amino acids 268-282 and 337-352 of human MBD4
Specificity This antibody detects MBD4.
Purification Protein G chromatography
Alternative Name MBD4
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (4). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (3). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (2). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (1).
Alternate names: MED1, Methyl-CpG-binding domain protein 4, Mismatch-specific DNA N-glycosylase
Gene ID 8930
NCBI Accession NP_003916.1
UniProt O95243
Research Area Chromatin and Nuclear Signaling
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).

Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Petronzelli, Riccio, Markham et al.: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

Bader, Walker, Harrison: "Most microsatellite unstable sporadic colorectal carcinomas carry MBD4 mutations." in: British journal of cancer, Vol. 83, Issue 12, pp. 1646-9, 2001 (PubMed).

Hosts (12), (3)
Reactivities (15), (5), (2)
Applications (15), (6), (1), (1)
Epitopes (1), (1), (1), (1)
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