Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (AA 300-350) antibody

Details for Product No. ABIN569372
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Antigen
Epitope
AA 300-350
(8), (7), (3), (3), (2), (1), (1)
Reactivity
Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Opossum (Didelphimorphia), Rat (Rattus)
(43), (26), (20), (19), (18), (17), (17), (2), (1), (1), (1)
Host
Rabbit
(37), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(19), (18), (15), (10), (7), (6), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN569372
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Immunogen Amino acids 300-350 of human RNF174 .Remarks: The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, CRA_b and CRA_c), mouse, rat, horse and chimpanzee, 94% homologous in dog and opossum, 82% homologous in chicken and 77% homologous in zebrafish.
Specificity This antibody detects MARCH4 / RNF174. Species Reactivity: Tested: Chimpanzee, Horse, Human, Mouse, Rat Expected from sequence similarity: Chicken, Dog, Opossum, Zebrafish
Purification Antigen affinity chromatography
Alternative Name MARCH4 / RNF174
Background RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv- type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.
Alternate names: E3 ubiquitin-protein ligase MARCH4, KIAA1399, MARCH-IV, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, RING finger protein 174
Gene ID 57574
NCBI Accession NP_065865
UniProt Q9P2E8
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% gelatin and 0.05% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

Gerhard, Wagner, Feingold et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

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