Second Mitochondria-Derived Activator of Caspase (DIABLO) (AA 226-239) antibody

Details for Product No. ABIN569418
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Antigen
Epitope
AA 226-239
(32), (17), (9), (9), (4), (3), (3), (3), (2), (2), (1), (1)
Reactivity
Human
(135), (56), (46), (15), (15), (14), (13), (2), (1)
Host
Rabbit
(119), (22), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(127), (49), (41), (37), (15), (15), (10), (6), (5), (4), (3), (3), (2), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN569418
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Immunogen Synthetic peptide corresponding to amino acids 226 to 239 of Human Smac
Specificity This antibody detects DIABLO / SMAC. Species Reactivity: Tested: Human.
Purification Protein G Chromatography
Alternative Name DIABLO / SMAC
Background Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa (3).
Alternate names: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 56616
NCBI Accession NP_063940
UniProt Q9NR28
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2% Gelatin and 0.05% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for six months or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Du, Fang, Li et al.: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000 (PubMed).

Srinivasula, Datta, Fan et al.: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." in: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, 2000 (PubMed).

Chai, Du, Wu et al.: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000 (PubMed).

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