Toll-Like Receptor 3 (TLR3) (Ligand Binding Domain) antibody

Details for Product No. ABIN569487
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Antigen
Epitope
Ligand Binding Domain
(27), (20), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Macaque, Cow (Bovine), Dog (Canine), Chimpanzee, Cat (Feline), Human, Monkey, Mouse (Murine), Opossum (Didelphimorphia), Rat (Rattus)
(177), (122), (63), (45), (12), (4), (1), (1), (1)
Host
Rabbit
(117), (90), (9), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(25), (11), (11), (9), (9), (8), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(135), (88), (65), (52), (32), (26), (19), (15), (14), (10), (8), (6), (5), (1), (1)
Pubmed 7 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN569487
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Immunogen Peptide containing H-539 and N-541 .Remarks: H-539 and N-541 are two important amino acids involved in the binding of dsRNA to TLR3. The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, macaque, orangutan, gorilla, chimpanzee, cow, and opossum, 95% homologous in dog and wild boar, 90% homologous in cat and rat, and 85% homologous in mouse.
Specificity This antibody detects CD283 / TLR3 (ligand binding domain). Species Reactivity: Tested: Chimpanzee, Cow, Gorilla, Human, Macaque, Mouse, Opossum, Rat. Expected from sequence similarity: Cat, Dog.
Purification Antigen Affinity Chromatography
Alternative Name CD283 / TLR3
Background The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) and lymph nodes (4,5). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. TLR3 is activated by dsRNA, the genetic material of some viruses, and a viral replication intermediate in others (6). Crystal struture and mutational analyses of TLR3-ectodomain suggest that Histidine-539 and Asparagine-541 are important amino acid residues in the recognition of dsRNA. H539E and N541A mutations abolish the dsRNA binding and ligand-dependent activation of TLR3 (7). This polyclonal antibody may block dsRNA binding to TLR3.
Alternate names: Toll-like receptor 3
Gene ID 9606
UniProt O15455
Research Area Signaling, Immunology, Inflammation
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS, 0.05% Sodium Azide 0.05% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Medzhitov, Janeway: "Innate immunity: the virtues of a nonclonal system of recognition." in: Cell, Vol. 91, Issue 3, pp. 295-8, 1997 (PubMed).

Rock, Hardiman, Timans et al.: "A family of human receptors structurally related to Drosophila Toll." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 588-93, 1998 (PubMed).

Muzio, Natoli, Saccani et al.: "The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6)." in: The Journal of experimental medicine, Vol. 187, Issue 12, pp. 2097-101, 1998 (PubMed).

Muzio, Polentarutti, Bosisio et al.: "Toll-like receptors: a growing family of immune receptors that are differentially expressed and regulated by different leukocytes." in: Journal of leukocyte biology, Vol. 67, Issue 4, pp. 450-6, 2000 (PubMed).

Muzio, Bosisio, Polentarutti et al.: "Differential expression and regulation of toll-like receptors (TLR) in human leukocytes: selective expression of TLR3 in dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5998-6004, 2000 (PubMed).

Alexopoulou, Holt, Medzhitov et al.: "Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3." in: Nature, Vol. 413, Issue 6857, pp. 732-8, 2001 (PubMed).

Bell, Askins, Hall et al.: "The dsRNA binding site of human Toll-like receptor 3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 23, pp. 8792-7, 2006 (PubMed).

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