TSPO antibody (C-Term)

Catalog No. ABIN570960

Product Details anti-TSPO Antibody

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TSPO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: PBR (mouse)

Sequence: RDNSGRRGGS RLPE

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RDNSGRRGGSRLPE, from the C Terminus of the protein sequence according to NP_033905.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Mouse Kidney and Colon. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx.16-17 kDa band observed in Mouse Kidney lysates and in lysates of cell line NIH3T3 (calculated MW of 18.8 kDa according to NP_033905.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TSPO antibody (ABIN570960)

Morgan, Cheepala, Wang, Neale, Adachi, Nachagari, Leggas, Zhao, Boyd, Venkataramanan, Schuetz: "Deregulated hepatic metabolism exacerbates impaired testosterone production in Mrp4-deficient mice." in: The Journal of biological chemistry, Vol. 287, Issue 18, pp. 14456-66, (2012) (PubMed).

Target Details for TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Alternative Name: Tspo (TSPO Products)

Synonyms: LOC732875 antibody, LOC100136652 antibody, LOC100280949 antibody, BZRP antibody, TSPO antibody, Bzrp antibody, IBP antibody, PBR antibody, Tspo1 antibody, MBR antibody, PTBZR02 antibody, Ptbzr antibody, RATPTBZR02 antibody, BPBS antibody, DBI antibody, PBS antibody, PKBS antibody, PTBR antibody, mDRC antibody, pk18 antibody, peripheral-type benzodiazepine receptor antibody, Peripheral-type benzodiazepine receptor antibody, putative peripheral-type benzodiazepine receptor antibody, translocator protein antibody, translocator protein homolog antibody, hypothetical protein antibody, LOC732875 antibody, MAV_3896 antibody, xccb100_2086 antibody, Smp_102510 antibody, Sros_1911 antibody, tspo antibody, LOC100280949 antibody, PGTG_16395 antibody, TSPO antibody, Tspo antibody

Background: PBR, Tspo, translocator protein, Bzrp, IBP, benzodiazepine receptor, peripheral, isoquinoline-binding protein, peripheral benzodiazepine receptor

Gene ID: 12257, 24230

NCBI Accession: NP_033905

Pathways: Cellular Response to Molecule of Bacterial Origin