Histone H1 antibody
| Antigen |
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| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Clonality |
Monoclonal (AE-4) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN573964 |
| Quantity |
50 µg (1 mg/ml) |
| Price |
513.33 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Immunogen
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Cells, Nuclei from myeloid leukemia biopsy cells.
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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AE-4
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Description
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Gene: Histone H1
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Specificity
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Recognizes histone H1. The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber. In blots of rat brain homogenate a duplet is observed at 27-29 kD consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.
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Application Notes
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ELISA, IF, IHC-Fr, IHC-P (5 µg/ml), WB (1:100)
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Concentration
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1 mg/ml
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Purification
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Protein G Column
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Buffer
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PBS, 0.09% sodium azide.
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Storage
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+4°C or -20°C, Avoid repeated freezing and thawing.
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Research Area
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Chromatin and Nuclear Signaling, DNA/RNA, Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "Histone H1", type "Antibodies"
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Hosts
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Rabbit (28), Mouse (17), Chicken (4), Sheep (4)
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Reactivities
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Human (42), Cow (Bovine) (21), Mouse (Murine) (21), Rat (Rattus) (17), Dog (Canine) (16), Horse (Equine) (16), Rabbit (16), Pig (Porcine) (15)
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Applications
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Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunohistochemistry (IHC) (10), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Acetyl-Lys25 (1), pThr17 (1)
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