PDGF-BB Homodimer (AA 222-233) antibody

Catalog No. ABIN574108

Product Details

Target: PDGF-BB Homodimer

Binding Specificity: AA 222-233

Reactivity: Human, Cow, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PDGFB.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Sheep, Bovine, Opossum (100%) Marmoset, Mouse, Rat, Elephant, Panda, Bat, Dog, Horse (92%) Cat (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KHRKFKHTHDKT from C-Terminus of human PDGFB (NP_002599.1, NP_148937.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Sheep, Bovine, Opossum (100%), Marmoset, Mouse, Rat, Elephant, Panda, Bat, Dog, Horse (92%), Cat (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Synonyms: PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody

Background: Name/Gene ID: PDGFB
Family: PDGF

Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain

Gene ID: 5155

NCBI Accession: NP_002599, NP_148937

UniProt: P01127