Calnexin antibody (AA 204-218)

Catalog No. ABIN574328

Product Details anti-Calnexin Antibody

Target: Calnexin (CANX)

Binding Specificity: AA 204-218

Reactivity: Human, Mouse, Dog, Cow, Rabbit, Pig, Monkey, Chicken, Horse, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CANX / Calnexin. Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Chicken (100%) Rat, Hamster, Turkey, Platypus (93%) Marmoset, Mouse (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SKTPELNLDQFHDKT from an internal region (near N-terminus) of human CANX / Calnexin (NP_001737.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Elephant, Panda, Bovine, Bat, Dog, Horse, Rabbit, Pig, Chicken (100%), Rat, Hamster, Turkey, Platypus (93%), Marmoset, Mouse (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0.3 - 1 μg/mL)

Usage: Western Blot: Approx. 100kD band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kD according to Human NP_001737.1 and 67.2kD according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7,271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX / Calnexin (CANX Products)

Synonyms: CNX antibody, IP90 antibody, P90 antibody, 1110069N15Rik antibody, AI988026 antibody, Cnx antibody, D11Ertd153e antibody, canx antibody, clgn antibody, clnx antibody, cnx antibody, PP90 antibody, zgc:63524 antibody, wu:fe06b12 antibody, CANX antibody, calnexin antibody, CalNeXin antibody, calnexin L homeolog antibody, CANX antibody, Canx antibody, cnx-1 antibody, canx.L antibody, canx antibody, LOC100283947 antibody

Background: Name/Gene ID: CANX

Synonyms: CANX, CNX, Calnexin, IP90, p90

Gene ID: 821

NCBI Accession: NP_001737

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis