DYKDDDDK Tag antibody (C-Term) (HRP)

Catalog No. ABIN595109

Product Details anti-DYKDDDDK Tag Antibody (HRP)

Target: DYKDDDDK Tag

Binding Specificity: C-Term

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Sequence: DYKDDDDK

Specificity: Recognizes DYKDDDDK when fused to either the amino or carboxyl terminus of target proteins. Shows greater binding affinity and sensitivity to fusion proteins containing the DYKDDDDK sequence. Does not cross-react with any endogenous proteins.

Purification: Affinity purified

Immunogen: Synthetic peptide corresponding to Enterokinase cleavage site, DYKDDDDK (KLH). Residues of cysteine and glycine were used to facilitate coupling at the C-terminal end.

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA and Western Blot. ELISA: 1:10000-1:50000. Western Blot: 1:1000-1:5000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2, 0.01 % Gentamicin sulfate 1 % BSA

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing. Sensitive to light.

Storage: -20 °C

Storage Comment: May be stored at -20°C lyophilized. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C. Labeled with horseradish peroxidase (HRP). Protect from light.

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Products

Target Type: Tag