TGF-beta antibody

Catalog No. ABIN604857

Product Details anti-TGF-beta Antibody

Target: TGF-beta (TGFb)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGF-beta antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut), ELISA, Functional Studies (Func), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes both human platelet-derived and recombinant TGF-beta.

Purification: Protein G purified

Immunogen: TGF-b from human platelets.

Type of Immunogen: Purified protein

Clone: TB21

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, Func, IHC, IHC-Fr, IHC-P (10 μg/mL), Neut, WB

Usage: Suitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

References for anti-TGF-beta antibody (ABIN604857)

Baumeier, Escher, Aleshcheva, Pietsch, Schultheiss: "Plasminogen activator inhibitor-1 reduces cardiac fibrosis and promotes M2 macrophage polarization in inflammatory cardiomyopathy." in: Basic research in cardiology, Vol. 116, Issue 1, pp. 1, (2021) (PubMed).

Target Details for TGF-beta

Target: TGF-beta (TGFb)

Alternative Name: TGF-beta (TGFb Products)

Synonyms: CED antibody, DPD1 antibody, LAP antibody, TGFB antibody, TGFbeta antibody, TGF-beta antibody, TGF-BETA-1 antibody, TGF-beta1 antibody, TGFbeta1 antibody, Tgfb antibody, Tgfb-1 antibody, transforming growth factor beta 1 antibody, transforming growth factor beta-1 antibody, transforming growth factor, beta 1 antibody, TGFB1 antibody, Tgfb1 antibody

Pathways: EGFR Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus