SELPLG antibody (Selectin P Ligand) (APC)

Details for Product anti-SELPLG Antibody No. ABIN609651, Supplier: Log in to see
Antigen
  • SELPLG
  • SELPG
  • CD162
  • CLA
  • PSGL-1
  • PSGL1
  • Psgl1
  • Selpl
  • Psgl-1
  • Selp1
  • 9030417L18
  • Slp1
  • selectin P ligand
  • clasper
  • selectin, platelet (p-selectin) ligand
  • synaptotagmin-like 1
  • SELPLG
  • cla
  • Selplg
  • Sytl1
Reactivity
Human
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36
22
13
1
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1
Host
Mouse
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85
9
Clonality (Clone)
Monoclonal ()
Conjugate
This SELPLG antibody is conjugated to APC
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3
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2
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2
Application
Flow Cytometry (FACS)
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86
62
34
29
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23
21
13
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Options
Supplier
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Supplier Product No.
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Immunogen Human thymocytes
Clone TC2
Isotype IgG1
Specificity The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on a subpopulation of B lymphocytes and CD34+ bone marrow cells.
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name SELPLG (SELPLG Antibody Abstract)
Background CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
Gene ID 6404
Research Area Atherosclerosis, Surface Receptors of Immune Cells, CD Antigens, Cardiovascular
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-Selectin P Ligand (SELPLG) antibody (APC) (ABIN609651) Surface staining of human peripheral blood cells with anti-human CD162 (clone TC2).
Background publications Hayden-Ledbetter, Cerveny, Espling, Brady, Grosmaire, Tan, Bader, Slater, Nilsson, Barone, Simon, Bradley, Thompson, Wahl, Ledbetter: "CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 8, pp. 2739-46, 2009 (PubMed).

Diehl, Schmidlin, Nagasawa, van Haren, Kwakkenbos, Yasuda, Beaumont, Scheeren, Spits: "STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4805-15, 2008 (PubMed).

Marsik, Mayr, Cardona, Schaller, Wagner, Jilma: "Endotoxin down-modulates P-selectin glycoprotein ligand-1 (PSGL-1, CD162) on neutrophils in humans." in: Journal of clinical immunology, Vol. 24, Issue 1, pp. 62-5, 2004 (PubMed).

Polyak, Ayer, Szczepek, Deans: "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody." in: Leukemia, Vol. 17, Issue 7, pp. 1384-9, 2003 (PubMed).

Chan, Hughes, French, Tutt, Walshe, Teeling, Glennie, Cragg: "CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts." in: Cancer research, Vol. 63, Issue 17, pp. 5480-9, 2003 (PubMed).

Rose, Smith, Maloney: "Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects." in: Blood, Vol. 100, Issue 5, pp. 1765-73, 2002 (PubMed).

Polyak, Deans et al.: "Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements ..." in: Blood, Vol. 99, Issue 9, pp. 3256-62, 2002 (PubMed).

Davenpeck, Brummet, Hudson, Mayer, Bochner: "Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 5, pp. 2764-72, 2000 (PubMed).

Deans, Robbins, Polyak, Savage: "Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 344-8, 1998 (PubMed).

Moore: "Structure and function of P-selectin glycoprotein ligand-1." in: Leukemia & lymphoma, Vol. 29, Issue 1-2, pp. 1-15, 1998 (PubMed).

Deans, Kalt, Ledbetter, Schieven, Bolen, Johnson: "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20." in: The Journal of biological chemistry, Vol. 270, Issue 38, pp. 22632-8, 1995 (PubMed).

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