Nanog Homeobox (NANOG) (AA 135-184) antibody

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Antigen
  • 2410002E02Rik
  • ENK
  • ecat4
  • Nanog homeobox
  • Nanog
  • NANOG
Alternatives
anti-Human Nanog Homeobox antibody for Immunofluorescence (fixed cells)
Epitope
AA 135-184
35
27
25
21
19
16
16
15
15
14
14
13
10
9
7
5
5
4
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Monkey, Sheep (Ovine)
377
136
44
8
6
6
5
4
4
2
2
1
1
1
Host
Rabbit
262
106
44
2
Clonality
Polyclonal
Conjugate
Un-conjugated
18
18
18
17
12
12
7
6
5
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
Application
Western Blotting (WB)
308
204
143
102
78
77
70
36
26
13
12
7
4
4
3
2
1
1
1
1
1
Options
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Immunogen Synthetic peptide located between aa135-184 of human NANOG (Q6NSW7, NP_079141). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Dog, Cat, Horse, Pig, Opossum (92%), Sheep, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide
Specificity Human Nanog
Predicted Reactivity Percent identity by BLAST analysis: Human (100%) Mouse, Rat, Dog, Pig (92%) Sheep, Bovine (85%).
Purification Immunoaffinity purified
Alternative Name NANOG (NANOG Antibody Abstract)
Background Name/Gene ID: NANOG

Synonyms: NANOG, Nanog homeobox, NANOGP8, HNanog, Homeobox protein NANOG
Gene ID 79923
NCBI Accession NP_079141
UniProt Q9H9S0
Pathways Stem Cell Maintenance
Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Supplier Images
 image for anti-Nanog Homeobox (NANOG) (AA 135-184) antibody (ABIN610568) anti-Nanog Homeobox (NANOG) (AA 135-184) antibody
Product cited in: Ku, Meier: "Phosphorylation of paxillin via the ERK mitogen-activated protein kinase cascade in EL4 thymoma cells." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11333-40, 2000 (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, 1999 (PubMed).

Salgia, Li, Lo, Brunkhorst, Kansas, Sobhany, Sun, Pisick, Hallek, Ernst: "Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL." in: The Journal of biological chemistry, Vol. 270, Issue 10, pp. 5039-47, 1995 (PubMed).