BBS5 antibody (N-Term)

Catalog No. ABIN6746105

Product Details anti-BBS5 Antibody

Target: BBS5 (Bardet-Biedl Syndrome 5 (BBS5))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit, Dog, Monkey, Pig, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BBS5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human BBS5

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human BBS5 (Q8N3I7, NP_689597). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Catfish, Salmon, Smelt, Stickleback, Zebrafish (100%), Gorilla, Bovine, Zebra finch, Beetle (92%), Sea squirt (90%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-BBS5 antibody (ABIN6746105)

Charron, Nakamura, Bacallao, Wandinger-Ness: "Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 111-24, (2000) (PubMed).

Ting, Hazuka, Hsu, Kirk, Bean, Scheller: "rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9613-7, (1995) (PubMed).

Target Details for BBS5

Target: BBS5 (Bardet-Biedl Syndrome 5 (BBS5))

Alternative Name: BBS5 (BBS5 Products)

Synonyms: zgc:56578 antibody, 1700049I01Rik antibody, 2700023J09Rik antibody, Bardet-Biedl syndrome 5 antibody, Bardet-Biedl syndrome 5 (human) antibody, bbs5 antibody, BBS5 antibody, Bbs5 antibody

Background: Name/Gene ID: BBS5

Synonyms: BBS5, Bardet-Biedl syndrome 5

Gene ID: 129880

NCBI Accession: NP_689597

UniProt: Q8N3I7

Pathways: Hedgehog Signaling