BCAT1 antibody (Branched Chain Amino-Acid Transaminase 1, Cytosolic) (C-Term)

Details for Product anti-BCAT1 Antibody No. ABIN610836, Supplier: Log in to see
Antigen
  • fj66g02
  • zgc:73157
  • wu:fj66g02
  • Bcatc
  • BCATC
  • BCT1
  • ECA39
  • MECA39
  • PNAS121
  • PP18
  • BCATc
  • Eca39
  • branched chain aminotransferase 1, cytosolic
  • branched chain amino-acid transaminase 1, cytosolic
  • branched chain amino acid transaminase 1, cytosolic
  • bcat1
  • BCAT1
  • Bcat1
Epitope
C-Term
11
11
10
9
9
6
6
5
3
3
2
2
1
1
1
1
1
Reactivity
Guinea Pig, Human, Monkey, Mouse (Murine), Rat (Rattus)
87
42
31
12
8
7
7
6
5
2
2
1
1
Host
Rabbit
76
17
Clonality
Polyclonal
Conjugate
This BCAT1 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
75
37
29
16
13
5
3
3
3
Options
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Immunogen Synthetic peptide from C-Terminus of mouse Bcat1 (Q8CBC8, NP_001019639). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Platypus (100%), Galago, Sheep, Dog, Bovine, Bat, Rabbit, Horse (92%), Opossum, Turkey, Chicken (85%), Rat (81%).

Type of Immunogen: Synthetic peptide
Specificity Mouse BCAT1
Predicted Reactivity Percent identity by BLAST analysis: Human (100%) Sheep, Dog, Bovine, Rabbit, Horse (92%) Mouse, Chicken (85%) Rat (81%).
Purification Immunoaffinity purified
Alternative Name BCAT1 / ECA39 (BCAT1 Antibody Abstract)
Background Name/Gene ID: BCAT1

Synonyms: BCAT1, BCAT(c), BCATC, ECA39, MECA39, BCT1, Placental protein 18, PNAS121, PP18, Protein ECA39
Gene ID 586
NCBI Accession NP_001019639
UniProt P54687
Application Notes Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Supplier Images
 image for anti-BCAT1 antibody (Branched Chain Amino-Acid Transaminase 1, Cytosolic) (C-Term) (ABIN610836) anti-Branched Chain Amino-Acid Transaminase 1, Cytosolic (BCAT1) (C-Term) antibody
Product cited in: Nakatani, Thompson, Barthel, Sakaue, Liu, Weigel, Roth: "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21528-32, 1999 (PubMed).

Frech, Andjelkovic, Ingley, Reddy, Falck, Hemmings: "High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8474-81, 1997 (PubMed).

Cross, Alessi, Cohen, Andjelkovich, Hemmings: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996 (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 (PubMed).