TRKB antibody (AA 466-515)

Catalog No. ABIN6746328

Product Details anti-TRKB Antibody

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Binding Specificity: AA 466-515

Reactivity: Human, Monkey, Rabbit, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRKB antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human NTRK2 / TRKB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rabbit, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa466-515 of human NTRK2 (Q8WXJ5, NP_001018075). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Rabbit, Horse (100%), Bat (87%), Galago (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-TRKB antibody (ABIN6746328)

Ktistaki, Talianidis: "Modulation of hepatic gene expression by hepatocyte nuclear factor 1." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 109-12, (1997) (PubMed).

Kritis, Ktistaki, Barda, Zannis, Talianidis: "An indirect negative autoregulatory mechanism involved in hepatocyte nuclear factor-1 gene expression." in: Nucleic acids research, Vol. 21, Issue 25, pp. 5882-9, (1994) (PubMed).

Kuo, Conley, Hsieh, Francke, Crabtree: "Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9838-42, (1991) (PubMed).

Frain, Swart, Monaci, Nicosia, Stämpfli, Frank, Cortese: "The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain." in: Cell, Vol. 59, Issue 1, pp. 145-57, (1989) (PubMed).

Target Details for TRKB

Target: TRKB (NTRK2) (Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))

Alternative Name: NTRK2 / TRKB (NTRK2 Products)

Synonyms: trkb antibody, trkb-b antibody, xtrkb antibody, si:ch211-220b11.1 antibody, trkB2 antibody, NTRK2 antibody, ntrk2 antibody, gp145-trkb antibody, TRKB antibody, GP145-TrkB antibody, trk-B antibody, AI848316 antibody, C030027L06Rik antibody, Tkrb antibody, trkB antibody, RATTRKB1 antibody, TRKB1 antibody, Trk-B antibody, TrkB antibody, neurotrophic receptor tyrosine kinase 2 antibody, neurotrophic receptor tyrosine kinase 2 L homeolog antibody, neurotrophic tyrosine kinase, receptor, type 2b antibody, neurotrophic receptor tyrosine kinase 2 S homeolog antibody, neurotrophic tyrosine kinase, receptor, type 2 antibody, NTRK2 antibody, ntrk2.L antibody, ntrk2b antibody, ntrk2.S antibody, ntrk2 antibody, Ntrk2 antibody

Background: Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B

Gene ID: 4915

NCBI Accession: NP_001018075

UniProt: Q16620

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport