WASF2 antibody (AA 146-195)

Catalog No. ABIN6746329

Product Details anti-WASF2 Antibody

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Binding Specificity: AA 146-195

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Monkey, Pig, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASF2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse WASF2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Guinea pig (100%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa146-195 of mouse Wasf2 (Q8BH43, NP_700472). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Elephant, Turkey, Zebra finch, Xenopus (92%), Stickleback (91%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-WASF2 antibody (ABIN6746329)

Ktistaki, Talianidis: "Modulation of hepatic gene expression by hepatocyte nuclear factor 1." in: Science (New York, N.Y.), Vol. 277, Issue 5322, pp. 109-12, (1997) (PubMed).

Kritis, Ktistaki, Barda, Zannis, Talianidis: "An indirect negative autoregulatory mechanism involved in hepatocyte nuclear factor-1 gene expression." in: Nucleic acids research, Vol. 21, Issue 25, pp. 5882-9, (1994) (PubMed).

Kuo, Conley, Hsieh, Francke, Crabtree: "Molecular cloning, functional expression, and chromosomal localization of mouse hepatocyte nuclear factor 1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9838-42, (1991) (PubMed).

Frain, Swart, Monaci, Nicosia, Stämpfli, Frank, Cortese: "The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain." in: Cell, Vol. 59, Issue 1, pp. 145-57, (1989) (PubMed).

Target Details for WASF2

Target: WASF2 (WAS Protein Family, Member 2 (WASF2))

Alternative Name: SCAR2 / WAVE2 (WASF2 Products)

Background: Name/Gene ID: WASF2

Synonyms: WASF2, DJ393P12.2, IMD2, Protein WAVE-2, WASF4, WAVE2, SCAR2, WASP family protein member 2, WASP family protein member 4, WAS protein family, member 2

Gene ID: 10163

NCBI Accession: NP_700472

UniProt: Q9Y6W5

Pathways: RTK Signaling