FRZB antibody (AA 59-108)

Catalog No. ABIN6746384

Product Details anti-FRZB Antibody

Target: FRZB (Frizzled-Related Protein (FRZB))

Binding Specificity: AA 59-108

Reactivity: Human, Rat, Horse, Cow, Guinea Pig, Rabbit, Bat, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FRZB antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human FRZB

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Horse, Pig, Xenopus (100%) Mouse, Zebrafish (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa59-108 of human FRZB (Q92765, NP_001454). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Xenopus (100%), Mouse, Zebrafish (92%), Hamster, Zebra finch, Chicken (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-FRZB antibody (ABIN6746384)

Kryl, Yacoubian, Haapasalo, Castren, Lo, Barker: "Subcellular localization of full-length and truncated Trk receptor isoforms in polarized neurons and epithelial cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 14, pp. 5823-33, (1999) (PubMed).

Gomeza, Joly, Kuhn, Knöpfel, Bockaert, Pin: "The second intracellular loop of metabotropic glutamate receptor 1 cooperates with the other intracellular domains to control coupling to G-proteins." in: The Journal of biological chemistry, Vol. 271, Issue 4, pp. 2199-205, (1996) (PubMed).

Tanabe, Masu, Ishii, Shigemoto, Nakanishi: "A family of metabotropic glutamate receptors." in: Neuron, Vol. 8, Issue 1, pp. 169-79, (1992) (PubMed).

Masu, Tanabe, Tsuchida, Shigemoto, Nakanishi: "Sequence and expression of a metabotropic glutamate receptor." in: Nature, Vol. 349, Issue 6312, pp. 760-5, (1991) (PubMed).

Target Details for FRZB

Target: FRZB (Frizzled-Related Protein (FRZB))

Alternative Name: SFRP-3 / FRZB (FRZB Products)

Synonyms: Frp antibody, Sfrp3 antibody, frezzled antibody, fritz antibody, frzb-1 antibody, FRE antibody, FRITZ antibody, FRP-3 antibody, FRZB-1 antibody, FRZB-PEN antibody, FRZB1 antibody, FZRB antibody, OS1 antibody, SFRP3 antibody, SRFP3 antibody, hFIZ antibody, Frzb-1 antibody, frizzled-related protein antibody, Frzb antibody, FRZB antibody

Background: Name/Gene ID: FRZB
Subfamily: Frizzled

Synonyms: FRZB, FRZB-1, FIZ, FRE, FRITZ, Fritz and frizzled, Frizzled homolog-related, FRP-3, FRZB-PEN, HFIZ, FRP, FZRB, SFRP-3, SRFP3, OS1, Frezzled, Frizzled related protein 3, Frizzled-related protein, Frizzled-related protein 1, FRZB1, SFRP3

Gene ID: 2487

NCBI Accession: NP_001454

UniProt: Q92765

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation