IER5L antibody (AA 331-380)

Catalog No. ABIN6746430

Product Details anti-IER5L Antibody

Target: IER5L (Immediate Early Response 5-Like (IER5L))

Binding Specificity: AA 331-380

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IER5L antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse IER5L

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Guinea pig (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa331-380 of mouse Ier5l (Q99J55, NP_084520). Percent identity by BLAST analysis: Human, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Bat, Guinea pig (100%), Elephant, Opossum (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-IER5L antibody (ABIN6746430)

Bodary, McLean: "The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line." in: The Journal of biological chemistry, Vol. 265, Issue 11, pp. 5938-41, (1990) (PubMed).

Suzuki, Argraves, Arai, Languino, Pierschbacher, Ruoslahti: "Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs." in: The Journal of biological chemistry, Vol. 262, Issue 29, pp. 14080-5, (1987) (PubMed).

Suzuki, Argraves, Pytela, Arai, Krusius, Pierschbacher, Ruoslahti: "cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 22, pp. 8614-8, (1986) (PubMed).

Target Details for IER5L

Target: IER5L (Immediate Early Response 5-Like (IER5L))

Alternative Name: IER5L (IER5L Products)

Background: Name/Gene ID: IER5L

Synonyms: IER5L, BA247A12.2

Gene ID: 389792

NCBI Accession: NP_084520

UniProt: Q5T953