NPFFR2 antibody (AA 251-300)

Catalog No. ABIN6746611

Product Details anti-NPFFR2 Antibody

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Binding Specificity: AA 251-300

Reactivity: Human, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPFFR2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human NPFFR2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Horse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa251-300 of human NPFFR2 (A0PJM9, NP_444264). Percent identity by BLAST analysis: Human (100%), Gorilla, Gibbon (92%), Monkey (85%), Horse (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-NPFFR2 antibody (ABIN6746611)

Pan, Thomson: "Nanog and transcriptional networks in embryonic stem cell pluripotency." in: Cell research, Vol. 17, Issue 1, pp. 42-9, (2007) (PubMed).

Nishimoto, Fukushima, Okuda, Muramatsu: "The gene for the embryonic stem cell coactivator UTF1 carries a regulatory element which selectively interacts with a complex composed of Oct-3/4 and Sox-2." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5453-65, (1999) (PubMed).

Vigano, Staudt: "Transcriptional activation by Oct-3: evidence for a specific role of the POU-specific domain in mediating functional interaction with Oct-1." in: Nucleic acids research, Vol. 24, Issue 11, pp. 2112-8, (1996) (PubMed).

Yuan, Corbi, Basilico, Dailey: "Developmental-specific activity of the FGF-4 enhancer requires the synergistic action of Sox2 and Oct-3." in: Genes & development, Vol. 9, Issue 21, pp. 2635-45, (1995) (PubMed).

Okamoto, Okazawa, Okuda, Sakai, Muramatsu, Hamada: "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells." in: Cell, Vol. 60, Issue 3, pp. 461-72, (1990) (PubMed).

Target Details for NPFFR2

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Alternative Name: NPFF2 / NPFFR2 (NPFF2 Products)

Synonyms: GPR74 antibody, HLWAR77 antibody, NPFF2 antibody, NPGPR antibody, Gpr74 antibody, HG31 antibody, Npff2 antibody, Npgpr antibody, neuropeptide FF receptor 2 antibody, NPFFR2 antibody, Npffr2 antibody

Background: Name/Gene ID: NPFFR2
Subfamily: Neuropeptide
Family: GPCR

Synonyms: NPFFR2, G protein-coupled receptor 74, HLWAR77, G-protein coupled receptor 74, Neuropeptide FF receptor 2, Neuropeptide FF 2, NPFF2, Neuropeptide FF 2 receptor, NPGPR, GPR74, NPFF-R2, NPFF2 receptor, NPGP receptor

Gene ID: 10886

NCBI Accession: NP_444264

UniProt: Q9Y5X5

Pathways: cAMP Metabolic Process