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Vinculin antibody (VCL) (AA 873-922)

Details for Product anti-VCL Antibody No. ABIN611567, Supplier: Log in to see
Antigen
  • VCL
  • im:6902790
  • hm:zehn2160
  • DDBDRAFT_0186762
  • DDBDRAFT_0232320
  • DDB_0186762
  • DDB_0232320
  • CG3299
  • CT11081
  • Dmel\\CG3299
  • Dmvincp
  • EG:103B4.1
  • Vin
  • Vin2EF
  • vinc
  • LOC100228062
  • CMD1W
  • CMH15
  • MV
  • MVCL
  • 9430097D22
  • AA571387
  • AI462105
  • AW545629
  • vcl-A
  • vinculin
  • vinculin A
  • Vinculin
  • VCL
  • vcl
  • vinA
  • Vinc
  • CpipJ_CPIJ013873
  • LOC100228062
  • LOC100356093
  • Vcl
  • Bm1_37935
Epitope
AA 873-922
24
15
9
8
7
6
6
6
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
184
94
79
35
10
7
5
3
3
3
3
2
2
2
1
1
1
1
Host
Rabbit
127
63
1
Clonality
Polyclonal
Conjugate
This Vinculin antibody is un-conjugated
8
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
145
65
59
53
46
22
21
19
13
13
6
5
4
1
1
1
1
Options
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Immunogen Synthetic peptide located between aa873-922 of human VCL (P18206, NP_003364). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Seabass, Zebrafish (100%), Xenopus (92%).

Type of Immunogen: Synthetic peptide
Specificity Human VCL / Vinculin
Predicted Reactivity Percent identity by BLAST analysis: Human, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Zebrafish (100%).
Purification Immunoaffinity purified
Alternative Name VCL / Vinculin (VCL Antibody Abstract)
Background Name/Gene ID: VCL
Family: Cytoskeleton

Synonyms: VCL, CMD1W, CMH15, Metavinculin, Vinculin, MVCL
Gene ID 7414
NCBI Accession NP_003364
UniProt P18206
Pathways Cell-Cell Junction Organization
Application Notes Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Supplier Images
 image for anti-Vinculin antibody (VCL) (AA 873-922) (ABIN611567) anti-Vinculin (VCL) (AA 873-922) antibody
Product cited in: Mikita, Campbell, Wu, Williamson, Schindler: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Hou, Schindler, Henzel, Ho, Brasseur, McKnight: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Background publications Quelle, Shimoda, Thierfelder, Fischer, Kim, Ruben, Cleveland, Pierce, Keegan, Nelms: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).