CD28 (CD28) antibody

Antigen: CD28 (CD28)

Synonyms: Tp44, MGC138290

Clonality: Monoclonal (JJ319)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Catalog no.: ABIN611634

Additional Information

Alternative name: CD28

Gene ID: 25660

Immunogen: Mouse A20J B lymphoma cells transfected with rat CD28

Isotype: IgG1  (Matching secondary antibodies)

Clone: JJ319

Specificity: The mouse monoclonal antibody JJ319 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Application Details

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Tacke, Hanke, Hanke et al.: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, 1997 (PubMed).

Dengler, Szabo, Sido et al.: "Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation." in: Transplantation, Vol. 67, Issue 3, pp. 392-8, 1999 (PubMed).

Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, 2001 (PubMed).

Laskowski, Pratschke, Wilhelm et al.: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, 2002 (PubMed).

Haspot, Villemain, Laflamme et al.: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, 2002 (PubMed).

Lühder, Huang, Dennehy et al.: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, 2003 (PubMed).

van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, 2004 (PubMed).

Kerstan, Armbruster, Leverkus et al.: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, 2006 (PubMed).

Guillonneau, Séveno, Dugast et al.: "Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8164-71, 2007 (PubMed).