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Chicken anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 800)
Heavy & Light Chain
Alternatives DyLight 800
Flow Cytometry (FACS), Immunofluorescence (IF)
|4 references available|
|Quantity||1 mg (1 mg/ml (E 1% at 280 nm = 13.2)) (Variants)|
|Price||190.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||Purified Goat IgG, whole molecule|
|Description||Chicken anti-Goat IgG (H&L) - Affinity Pure, DyLight 800 Conjugate. Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin. Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm). Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.|
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
|Application Notes||This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications|
|Concentration||1 mg/ml (E 1% at 280 nm = 13.2)|
|Purity||Affinity purified antibody is > 95% based on SDS-PAGE|
|Purification||Affinity purified using solid phase mouse IgG (H&L)|
|Buffer||10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide|
|Storage||Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.|
|Research Area||Secondary Antibodies|
|Restrictions||For Research Use only|
Kirschner, Copeland, Gilbert et al.: "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." in: Genomics, Vol. 24, Issue 2, pp. 218-24, 1995 (PubMed).
Tanaka, Watase, Manabe et al.: "Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1." in: Science (New York, N.Y.), Vol. 276, Issue 5319, pp. 1699-702, 1997 (PubMed).
Yamada, Watanabe, Shibata et al.: "Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 15, pp. 5706-13, 1998 (PubMed).
Grunewald, Kanner: "The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9684-9, 2000 (PubMed).