Chicken anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 800)

Antigen: IgG

Binding Site: Heavy & Light Chain

Host: Chicken

Reactivity: Goat

Conjugate: DyLight 800

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Catalog no.: ABIN611654

Additional Information

Immunogen: Purified Goat IgG, whole molecule

Format: Lyophilized

Description: Chicken anti-Goat IgG (H&L) - Affinity Pure, DyLight 800 Conjugate. Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin. Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm). Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.

Comments:

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy, flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Concentration: 1 mg/ml (E 1% at 280 nm = 13.2)

Purity: Affinity purified antibody is > 95% based on SDS-PAGE

Purification: Affinity purified using solid phase mouse IgG (H&L)

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide

Storage: Store freeze-dried powder at 2-8 C. Shelf Life: Product is stable for up to 4 weeks at 2-8 C after rehydration. For extended storage after rehydration, add an equal volume of glycerol, store at -20 C. Reconstitution: Rehydrate with 1.1 ml of deionized water, let stand at room temperature to dissolve. Centrifuge to remove any particulates Prepare fresh working dilution daily.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Product: Kirschner, Copeland, Gilbert et al.: "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." in: Genomics, Vol. 24, Issue 2, pp. 218-24, 1995 (PubMed).

Tanaka, Watase, Manabe et al.: "Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1." in: Science (New York, N.Y.), Vol. 276, Issue 5319, pp. 1699-702, 1997 (PubMed).

Yamada, Watanabe, Shibata et al.: "Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 15, pp. 5706-13, 1998 (PubMed).

Grunewald, Kanner: "The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9684-9, 2000 (PubMed).