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Chicken anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 800)

Details for Product No. ABIN611654, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4586
1866
1790
962
821
643
618
279
35
34
31
8
8
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Goat
2753
2709
2346
1737
1451
749
638
448
383
356
356
349
315
263
165
150
130
113
41
27
21
21
12
8
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
Host
Chicken
6860
4762
1779
767
673
368
92
52
51
8
8
5
4
3
1
1
Conjugate
DyLight 800
2163
1852
1749
1326
718
631
532
292
283
228
222
218
217
217
176
117
81
79
69
54
49
39
37
25
23
19
19
18
15
15
15
14
14
14
14
13
11
11
11
10
10
10
10
10
9
9
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
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Immunogen Purified Goat IgG, whole molecule
Characteristics Chicken anti-Goat IgG (H&L) - Affinity Pure, DyLight 800 Conjugate.
Fluorphore: DyLight 800 (Ex = 777 nm, Em = 794 nm).
Fluor Protein Ratio: Moles DyLight 800 per Mole Antibody.
Purification Affinity purified using solid phase mouse IgG (H&L)
Purity > 95 % based on SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications
Comment

Country of Origin: Chicken Ig fraction was prepared using eggs from healthy hens of US origin.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions For Research Use only
Format Lyophilized
Concentration 1 mg/mL
Buffer 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Storage 4 °C
Product cited in: Grunewald, Kanner: "The accessibility of a novel reentrant loop of the glutamate transporter GLT-1 is restricted by its substrate." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9684-9, 2000

Yamada, Watanabe, Shibata, Nagashima, Tanaka, Inoue: "Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 15, pp. 5706-13, 1998

Tanaka, Watase, Manabe, Yamada, Watanabe, Takahashi, Iwama, Nishikawa, Ichihara, Kikuchi, Okuyama, Kawashima, Hori, Takimoto, Wada: "Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1." in: Science (New York, N.Y.), Vol. 276, Issue 5319, pp. 1699-702, 1997

Kirschner, Copeland, Gilbert, Jenkins, Amara: "Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2." in: Genomics, Vol. 24, Issue 2, pp. 218-24, 1995